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INSTRUMENTAL METHODS OF ANALYSIS

UNIT I
:

Basic principles, instrumentation and application of solvent extraction, ion exchange, electrophoresis, paper chromatography, TLC, GC, HPLC.

UNIT II:
Limitations of analytical methods accuracy and precision, classification and minimization of errors. Electromagnetic radiation spectrophotometry - interaction of radiation with different types of molecular energy, basic principles, instrumentation and applications of UV and Visible spectrophotometer, IR and NMR.

UNIT III:
Introduction, principle, instrumentation and environmental applications of flame photometer - AAS. Atomization flame atomization graphite furnace atomizers, application of AAS. Atomic Emission Spectroscopy- Instrumentation - quantitative analysis - direct reading spectrometers. Plasma excitation - flame excitation - laser excitation - chemical interferences - concentration range - Mass spectrophotometer.

UNIT IV:
Introduction, principle, instrumentation and application of Naphelometry - Turbidometry - Conductometry - Potentiometry - Ion Selective Electrodes.

UNIT V:
Rules for construction of diagram and graphs - types of diagrams and graphs - measure of centre value and dispersion - correlation - regression, test of significance t, X2 and ANOV A.

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