2005 Guru Gobind Singh Indraprastha University B Tech Biotechnology Laboratory Techniques in Biotechnology Question paper for exam preparation. Question paper for 2005 Guru Gobind Singh Indraprastha University B Tech Biotechnology Laboratory Techniques in Biotechnology Question paper, 2005 Guru Gobind Singh Indraprastha University B Tech Biotechnology Laboratory Techniques in Biotechnology Question paper. SiteMap
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2005 Guru Gobind Singh Indraprastha University B Tech Biotechnology Laboratory Techniques in Biotechnology Question paper

University Question Papers
2005 Guru Gobind Singh Indraprastha University B Tech Biotechnology Laboratory Techniques in Biotechnology Question paper
END-TERM EXAMINATION
SECOND SEMESTER [B.TECH.] - MAY 2005
Paper Code: BT 124 Subject: Laboratory Techniques in Biotechnology

Time : 3 Hours Maximum Marks :50
Note: Attempt all questions. All questions carry equal marks.

Q.1 Fill in the Blanks :
(a) Microtomy is a technique used for ____________
(b) X-ray crystallography plays an important role in ______________
(c) Isotope may be defined as ____________
(d) Magnetic beads are used for ____________
(e) Scintillation coc ktails are mostly made of ____________
(f) Void volume is defined as ____________
(g) SDS-PAGE separates proteins on the basis of
____________
(h) Circular Dichroism (CD) spectroscopy is used for
__________
(i) Protein molecules of different mass in a mixture can be
separated by ____________
(j) Full form of CAT-Scan is ____________
(k) The unit of magnetic field strength is ____________
(l) Half life of radioactive phosphorous is ____________
days.

Q.2 Describe in detail basic principles of sedimentation.
OR
Define centrifugation and discuss different kinds of
centrifugation techniques.

Q.3 What is chromatography and explain the principle behind
ion-exchange chromatography.
OR
Bring out the difference between Gas Liquid
Chromatography (GLC) and High Performance Liquid
Chromatography (HPLC). Why in some cases HPLC is
preferred over GLC.

Q.4 What is microtomy? Describe the principle behind rotary
microtomy. Explain how you would prepare specimen for
wax sectioning.
OR
Explain in detail the principle of isoelectric focusing (IEF).
Why in some cases IEF is preferred over SDS-PAGE

Q.5 Bring out the difference between absorption spectrum and
action spectrum. Give the principle of Nuclear Magnetic
Resonance (NMR).
OR
What are different methods of scintillation counting? Bring
out the advantages and disadvantages of each method.


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2005 Guru Gobind Singh Indraprastha University B Tech Biotechnology Laboratory Techniques in Biotechnology Question paper for exam preparation. Question paper for 2005 Guru Gobind Singh Indraprastha University B Tech Biotechnology Laboratory Techniques in Biotechnology Question paper, 2005 Guru Gobind Singh Indraprastha University B Tech Biotechnology Laboratory Techniques in Biotechnology Question paper